Search results for "Small animal"
showing 10 items of 50 documents
Oral administration of pharmacological doses of Vitamins C and E reduces reproductive fitness and impairs the ovarian and uterine functions of female…
2002
This study aims to ascertain whether oral administration of pharmacological doses of Vitamins C and E has any detrimental effect on reproductive fitness of female mice. We fed hybrid female mice from the first day of weaning a standard diet supplemented or not supplemented with pharmacological doses of Vitamins C and E. At the age of 28 weeks, we individually caged females with a male for the rest of their reproductive life. We performed a series of mating experiments to ascertain the number of oocytes ovulated and the potential for embryo development in vitro to the blastocyst stage and in vivo to Day 12 of gestation. The antioxidant diet decreased the frequency of litters, litter size, to…
Direct transfer of vitrified rabbit embryos.
1994
In this study we looked at the feasibility of transferring vitrified rabbit embryos directly into recipient does. Compacted morulae were vitrified in a solution of 20% ethylene glycol and 20% dimethyl sulfoxide. After thawing, and without step-wise diluted solution, the vitrified embryos were transferred into the recipient's uterine horns. Survival rate at birth differed from fresh rabbit embryos (40% vs 55%, P0.05). However, the percentage of does that delivered (94%) and the survival rate suggested this method is suitable for both storage and simple transfer of rabbit morulae.
Computer assisted morphometric analysis of ram sperm heads: Evaluation of different fixative techniques
1998
The recent development of automated systems for morphometric sperm head analysis has provided a series of objective parameters which have facilitated the standardization of morphological semen evaluation. This current work attempts to establish the optimum fixing conditions for the morphometric characterization of ram spermatozoa. Ejaculates were obtained from 5 Merino rams used for periodic collection of semen and were diluted at 1:50 with TEST medium. Air-dried smears were fixed either in ethanol-ether (1:1), 50% methanol, 2% glutaraldehyde or SUZA fixative, in which case the smear was pretreated with chloramine. The samples were then stained with commercial kit Hemacolor. Once the prepar…
Imaging of Orthotopic Glioblastoma Xenografts in Mice Using a Clinical CT Scanner: Comparison with Micro-CT and Histology
2016
Purpose There is an increasing need for small animal in vivo imaging in murine orthotopic glioma models. Because dedicated small animal scanners are not available ubiquitously, the applicability of a clinical CT scanner for visualization and measurement of intracerebrally growing glioma xenografts in living mice was validated. Materials and Methods 2.5x106 U87MG cells were orthotopically implanted in NOD/SCID/ᵞc-/- mice (n = 9). Mice underwent contrast-enhanced (300 μl Iomeprol i.v.) imaging using a micro-CT (80 kV, 75 μAs, 360° rotation, 1,000 projections, scan time 33 s, resolution 40 x 40 x 53 μm) and a clinical CT scanner (4-row multislice detector; 120 kV, 150 mAs, slice thickness 0.5 …
Biophotonics in veterinary medicine: the first steps toward clinical translation
2019
In this paper, we analyzed the current situation and the potential of biophotonics and biomedical optics in veterinary medicine. Promising optical techniques such as optical coherence tomography, pulse oximeter, and hyperspectral imaging have been clinically translated into human medicine. But even though human and small animal medicine share personalized and state-of-the-art approach, biophotonics remains rarely exploited in the canine and feline medicine. However, there are some biophotonics studies in veterinary oncology which addressed tumor diagnosis (skin and subcutaneous tumors), prognosis (lymphoma), and therapy (clear surgical margins). Visible and near-infrared spectroscopy served…
Canine pancytopoenia and hemophagocytic lymphohistiocytosis.
2018
Leishmaniasis and rickettsial diseases are endemic and common in Mediterranean countries and so, as in humans, these diseases could be an important cause of HLH in dogs and may respond to similar medications. In conclusion, we think that the diagnosis of HLH should be considered in dogs with pancytopenia and, in selected cases, immunosuppressive treatment might be warranted.
Validity and reliability of the CatWalk system as a static and dynamic gait analysis tool for the assessment of functional nerve recovery in small an…
2017
Introduction: A range of behavioral testing paradigms have been developed for the research of central and peripheral nerve injuries with the help of small animal models. Following any nerve repair strategy, improved functional outcome may be the most important evidence of axon regeneration. A novel automated gait analysis system, the CatWalk™, can measure dynamic as well as static gait patterns of small animals. Of most interest in detecting functional recovery are in particular dynamic gait parameters, coordination measures, and the intensity of the animals paw prints. This article is designed to lead to a more efficient choice of CatWalk parameters in future studies concerning the functio…
The turnover of dental microwear texture: Testing the" last supper" effect in small mammals in a controlled feeding experiment
2020
Dental microwear texture (DMT) analysis is commonly applied for dietary reconstruction of vertebrates. The temporal scale on which dietarily informative microscopic wear forms on enamel surfaces is crucial to infer dietary flexibility and seasonality. Microwear is assumed to form shortly before the individual's death, reflecting information pertaining to the last meals consumed (“last supper” effect). In primate feeding experiments, microwear features formed within hours, suggesting rates of turnover within one to two weeks. As DMT formation experiments testing the persistence of microwear three-dimensionally (textures) are still lacking, we test how quickly DMTs form and pr…
Image quality analysis of
2019
Background 44Sc has been increasingly investigated as a potential alternative to 68Ga in the development of tracers for positron emission tomography (PET). The lower mean positron energy of 44Sc (0.63 MeV) compared to 68Ga (0.83 MeV) can result in better spatial image resolutions. However, high-energy γ-rays (1157 keV) are emitted at high rates (99.9%) during 44Sc decay, which can reduce image quality. Therefore, we investigated the impact of these physical properties and performed an unbiased performance evaluation of 44Sc and 68Ga with different imaging phantoms (image quality phantom, Derenzo phantom, and three-rod phantom) on two preclinical PET scanners (Mediso nanoScan PET/MRI, Siemen…
A Volumetric Method for Quantification of Cerebral Vasospasm in a Murine Model of Subarachnoid Hemorrhage
2018
Subarachnoid hemorrhage (SAH) is a subtype of hemorrhagic stroke. Cerebral vasospasm that occurs in the aftermath of the bleeding is an important factor determining patient outcome and is therefore frequently taken as a study endpoint. However, in small animal studies on SAH, quantification of cerebral vasospasm is a major challenge. Here, an ex vivo method is presented that allows quantification of volumes of entire vessel segments, which can be used as an objective measure to quantify cerebral vasospasm. In a first step, endovascular casting of the cerebral vasculature is performed using a radiopaque casting agent. Then, cross-sectional imaging data are acquired by micro computed tomograp…